Cell cycle-dependent activation of rous sarcoma virus-infected stationary chicken cells: avian leukosis virus group-specific antigens and ribonucleic acid.
نویسندگان
چکیده
Stationary chicken embryo fibroblasts exposed to Rous sarcoma virus (RSV) remained stably infected for at least 5 days, but they did not release infectious virus or become transformed until after cell division. These infected stationary cells did not contain avian leukosis virus group-specific antigens or ribonucleic acid (RNA) hybridizable to deoxyribonucleic acid (DNA) made by the RSV endogenous RNA-directed DNA polymerase activity.
منابع مشابه
Evidence that avian tumour virus-immune chicken sera recognize only viral structural antigens on the surface of avian tumour virus-infected cells.
The specificity of the humoral response of chickens to avian tumour viruses (ATV) was investigated by reacting ATV-immune sera with Triton X-100 extracts of uninfected, infected and transformed chicken embryo fibroblasts. Analysis of these immune reactions by polyacrylamide gel electrophoresis revealed that avian leukosis virus-challenged and Rous sarcoma virus-challenged chickens recognized on...
متن کاملWidespread presence, in chickens, of DNA complementary to the RNA genome of avian leukosis viruses.
DNA-RNA hybridization experiments have demonstrated the widespread presence in chickens of DNA complementary to the RNA of avian myeloblastosis virus. All apparently normal chicken embryos, or adult chickens that were tested, contained viral DNA in amounts ranging from 1.7 to 4.6 viral genome equivalents per cell. Embryos that were negative or positive for the group-specific antigen of avian le...
متن کاملIndependent regulation of endogenous and exogenous avian RNA tumor virus genes.
3H-Labeled complementary DNA specific for the envelope glycoprotein (env) gene of avian leukosis-sarcoma viruses was isolated by selective nucleic acid hybridization techniques, and used to analyze the expression of the endogenous provirus. The endogenous provirus in certain cell types termed chicken helper factor positive (chf+) can synthesize the envelope glycoprotein. Env DNA sequences were ...
متن کاملThe murine leukemia-sarcoma virus complex.
The development of in vitro (tissue culture) assay systems for murine leukemia (MuLV)l" 2 and sarcoma (MSV)3-7 viruses, the demonstration of the defectiveness of the MSV genome,3 and its rescue with various MuLV's7 have brought out certain remarkable similarities between these viruses and those of the avian leukosisRous sarcoma complex.3' 7, 8, "a Subsequent studies of the murine viruses, which...
متن کاملComparison of the small RNAs of polymerase-deficient and polymerase-positive Rous sarcoma virus and another species of avian retrovirus.
The small RNAs contained in virions of avian leukosis and sarcoma viruses are a virus-specific subset of the total small RNA population of the host cell. The reverse transcriptase protein must be present in the budding virion for this selection to take place. Virions of the alpha form of the Bryan strain of Rous sarcoma virus, which lack detectable reverse transcriptase, incorporated an unselec...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of virology
دوره 10 1 شماره
صفحات -
تاریخ انتشار 1972